Author : Manssuor Abulkasim Ahmed, Girija Shankar Shukla and Harish Kumar Bajaj

Urinary tract infections (UTIs) are the second most common infections in community practise. Prevalence of UTI is an extremely in females than males, 40% to 50% of whom will suffer at least one clinical episode during their lifetime. This study therefore focuses on the detection and incidence of UTI among pregnant women. It also aimed to isolate and identify the organisms that causing UTI among pregnant women, and determined their susceptibility patterns to commonly used antimicrobial agents. A total of one hundred (100) midstream urine sample were collected from pregnant women at the aged ranges from 16 to 40 years attending to Sri Vijaya Hospital at Mehdipatnam , Hyderabad city India between June 2015 and December 2015. Early morning clean-catch midstream urine was collected from each pregnant woman into a wide-mouthed sterile screw capped container. With a calibrated micro-loop, 0.001 ml. of urine was cultured on to a Blood agar, MacConkey agar, EMB agar , Nutrient agar and Muller Hinton Agar plates. After overnight incubation at 37º C for 24 hours, A total of Thirty nine (39%) out of 100 patients had urinary tract infection . The bacterial isolates were identified based on colony morphology characteristics, Gram stain reaction and biochemical tests. the commonest isolated organism was S. aureus (43.53%), followed by E.coli (35.89 %), Klebsiella pneumonia (10.25%), Pseudomonas aeruginosa (7.69%) and lowest incidence of the isolates was Proteus spp (2.56%). More than 97% of the isolates were resistance to ampicillin (97.3%), followed by Netillin (84.2%), in other hand the most effective antibiotic was Amikacin which show (5.2%) of the isolates resistance, followed by the resistance of isolates to Cefotaxime and Gentamicin were each (21%).

Keywords: Bacterial agents, UTI, Antimicrobial susceptibility, Pregnant women, Urine culture