News Updates Friday 28th Oct 2016 :
  • Welcome to International Press Corporation, world's leading publishers, We have served more than 10000+ authors
  • Articles are invited in engineering, science, technology, management, industrial engg, biotechnology etc.
  • Paper submission last date of Sept/Oct 2016 issue is 28 Oct 2016, Submit online or at
  • Our journals are indexed in University of Regensburg Germany, Google Scholar, Cross Reference data bases
  • Applications for reviewers are invited and can be sent directly to concerned editor's mail

Temporal changes in mRNA expression of heat shock protein genes in mammary epithelial cells of riverine buffalo in response to heat stress in vitro

Author : Neha Kapila, Amit Kishore, Monika Sodhi, Ankita Sharma, AK Mohanty, Pawan Kumar and M Mukesh

Pages : 5-9
Download PDF

Exposure to high ambient temperature affects buffalo productivity significantly in hot climate areas. Till date, no data is available on the heat stress response of mammary epithelial cells (MECs) in buffaloes. The present work aimed to examine the long term temporal changes in mRNA expression pattern of various heat shock proteins (HSP) genes in buffalo MECs during heat stress condition. For the study, we utilized the primary mammary epithelial cells of riverine buffalo and were exposed to thermal stress condition at 420C for one hour. The cells were subsequently allowed to recover at 370C and harvested at different time intervals (30 min to 48 hr) along with control samples (un-stressed). All the HSP (HSP40, HSP60, HSP70, HSP90, and HSBP1) showed immediate induction in their expression after heat shock and remained upregulated at the later stages as well. Amongst these, HSP70 gene showed maximal induction in its expression while HSP40 was found to be second most abundantly expressed HSP. Our present data thus provides the strong clue about the responsiveness of buffalo MECs to heat stress suggesting its suitability as an in vitro model to understand the modulation of buffalo mammary gland expression signature in response to environmental heat load.

Keywords: Buffalo, cell culture, mammary epithelial cells, heat stress, heat shock protein.

Article published in Vol 3 (2013) in International Journal of Animal Biotechnology



Call for Papers
  1. IJCET- Sept/Oct-2016 Issue

    Submission Last Date
    28 Oct 2016
  2. IJTT-Sept-2016
  3. IJAIE-Sept-2016
  4. IJCSB-Sept-2016
  • Inpressco Google Scholar
  • Inpressco Science Central
  • Inpressco Global impact factor
  • Inpressco aap

International Press corporation is licensed under a Creative Commons Attribution-Non Commercial NoDerivs 3.0 Unported License
©2010-2016 INPRESSCO® All Rights Reserved