News Updates Saturday 21st Jul 2018 :
  • Welcome to INPRESSCO, world's leading publishers, We have served more than 10000+ authors
  • Articles are invited in engineering, science, technology, management, industrial engg, biotechnology etc.
  • Paper submission last date of July/Aug 2018 extended to 20 July 2018, Submit online or at editor.ijcet@inpressco.com
  • Our journals are indexed in NAAS, University of Regensburg Germany, Google Scholar, Cross Ref etc.
  • DOI is given to all articles

Targeting ceramidase and sphingosine kinase 1 of ceramide metabolic pathway induces apoptosis in human colon cancer cells


Author : Helen Shiphrah Vethakanraj, Mohamed Aslan S, Jayashree A, Vishnu Priya P and Ashok Kumar Sekar

Pages : 23-27, DOI: https://doi.org/10.14741/ijcsb/v.7.3
Download PDF
Abstract

The present study investigates the apoptotic effect of ceramidase inhibitor, Ceranib 2 and Sphingosine kinase 1 inhibitor, Dimethyl sphingosine in human colon cancer cell line SW480. Cells were treated with different doses of Ceranib 2 and Dimethyl sphingosine for 24, 48 and 72 hours to determine the cell viability using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT assay). Morphological changes were examined in phase contrast microscope, nuclear changes were observed using DAPI staining. DNA gel electrophoresis was performed to visualize DNA changes, Acridine orange/Ethidium bromide staining was done to discriminate live, early apoptotic, late apoptotic, necrotic cells. Mitochondrial membrane potential was determined by JC-1 staining. MTT assay revealed the cytotoxic effect of Ceranib 2 and Dimethyl sphingosine in SW480 cell line in dose and time dependant manner. Morphological changes and chromatin condensation observed in DAPI staining are morphological hallmarks of apoptosis. DNA ladder pattern observed in drug treated groups with respect to control group is biochemical hallmark of apoptosis. Further, presence of early and late apoptotic cells in Acridine orange/Ethidium bromide staining, loss of mitochondrial membrane potential in drug treated groups confirmed the apoptotic effect of Ceranib 2 and Dimethyl sphingosine in SW480 cells.

Keywords: Colon cancer, Apoptosis, Ceranib 2, Dimethyl sphingosine, Ceramidase, Sphingosine kinase 1

Call for Papers
  1. IJCET- July/Aug 2018 Issue

    Submission Last Date
    20 July
  2. DOI is given to all articles
  3. Current Issue
  4. IJTT-Sept-2018
  5. IJAIE-Sept-2018
  6. IJCSB-Sept-2018
  • Inpressco Google Scholar
  • Inpressco Science Central
  • Inpressco Global impact factor
  • Inpressco aap

International Press corporation is licensed under a Creative Commons Attribution-Non Commercial NoDerivs 3.0 Unported License
©2010-2018 INPRESSCO® All Rights Reserved