News Updates Sunday 22nd Dec 2024 :
  • Welcome to INPRESSCO, world's leading publishers, We have served more than 10000+ authors
  • Articles are invited in engineering, science, technology, management, industrial engg, biotechnology etc.
  • Paper submission is open. Submit online or at editor.ijcet@inpressco.com
  • Our journals are indexed in NAAS, University of Regensburg Germany, Google Scholar, Cross Ref etc.
  • DOI is given to all articles

Isolation, culturing and cryopreservation of putative amniotic fluid stem cells in sheep (Ovis aries)


Author : Anil Khuttan, Kapil Dev, Birbal Singh and Sanjeev K. Gautam

Pages : 10-13
Download PDF
Abstract

The current study was carried out to isolate, culture and cryopreserve the putative amniotic fluid stem (AFS) cells in sheep. AFS cells were cultured without feeder cells in DMEM containing 16% FBS, 1% penicillin/streptomycin and 1% L-glutamine in 5% CO2 in humidified air at 37±0.5˚C. After 6 days of culture different morphologies of cells were observed. Most of the cells started anchorage-dependent growth after day 7 of the culture. The putative AFS cells were cryopreserved using slow freezing method using DMSO as cryoprotectant. After freezing the cells for one week, it was observed that 70% of the cells were viable after trypan blue staining. This study shows that the putative amniotic fluid stem cells in sheep can be isolated, cultured and cryopreserved successfully under in vitro conditions.

Keywords: Amniotic fluid, stem cells, cryopreservation, sheep.

 

 

 

 

Call for Papers
  1. IJCET- Current Issue
  2. Issues are published in Feb, April, June, Aug, Oct and Dec
  3. DOI is given to all articles
  • Inpressco Google Scholar
  • Inpressco Science Central
  • Inpressco Global impact factor
  • Inpressco aap

International Press corporation is licensed under a Creative Commons Attribution-Non Commercial NoDerivs 3.0 Unported License
©2010-2023 INPRESSCO® All Rights Reserved