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Biological Approach of Zinc Oxide Nanoparticles Synthesis by Cell Free Extract of Spirulina Platensis


Author : Mohammad Irfan Ali, Gaurav Sharma, Manoj Kumar, Nakuleshwar Dut Jasuja, Rajgovind

Pages : 2531-2534
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Abstract

The present study explores biological synthesis of ZnO nanoparticles (ZnO NPs) using the cell free aqueous extract of Spirulina platensis. Biosynthesized ZnO NPs were characterized by UV-Vis spectroscopy, SEM, TEM, XRD and FTIR studies and finally tested for antibacterial activity. Bio-synthesis using extract of S. platensis showed the formation of well scattered, highly stable, spherical ZnO NPs with an average size of 40-50 nm. The size and morphology of the nanoparticles were confirmed by SEM and TEM analysis. FTIR and UV-Vis spectra showed that proteins and peptides are mainly responsible for the formation and stabilization of ZnO NPs. Furthermore, the synthesized nanoparticles exhibited good antibacterial activity against pathogenic gram-negative i.e. Escherichia Coli- MTCC-9721, Proteus vulgaris- MTCC-7299, Klebsiella pneumonia- MTCC-9751 and gram-positive i.e. Staphylococcus aureus- MTCC-9542, S. epidermidis- MTCC- 2639, Bacillus cereus- MTCC-9017 bacteria. The ZnO NPs had shown maximum zone of inhibition (ZOI) i.e. 34.8±1.65 in P. vulgaris. Use of such a biological method provides a simple, cost-effective alternative template for the synthesis of nanomaterials in a large scale that could be great use in biomedical applications.

Keywords: Zinc nanoparticles, Spirulina platensis, Antibacterial activity, and Biosynthesis

Article published in International Journal of Current Engineering and Technology, Vol.5, No.4 (Aug-2015)

 

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